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KMID : 1177720140080010037
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Journal of Alternatives to Animal Experiments
2014 Volume.8 No. 1 p.37 ~ p.42
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In Vitro Anti-dandruff Activity Test for Shampoo Formulation
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Kim So-Hee
Yun Yu-Na
Park Tae-Hoon
Lee John-Hwan
An Su-Sun
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Abstract
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Dandruff is a common scalp disorder that affects almost half of population. Cosmetic companies have developed anti-dandruff shampoo containing antimicrobial materials such as zinc pyrithione (ZPT) and climbazole which inhibit fungal growth especially Malassezia yeast, dandruff-causing organism. It is essential to examine whether anti-dandruff shampoo reduces Malassezia growth, so that improve dandruff. The aim of this investigation was to establish a test method for antifungal activity of shampoo. The basic methods of antimicrobial susceptibility testing are the minimum inhibitory concentration (MIC) and disk diffusion test. We have compared a disk diffusion test that uses nitrocellulose filter paper and artificial skin substrate (Vitro-Skin) as disks to estimate antifungal activity of shampoo or materials after rinse-off. We have identified that ZPT, climbazole and three commercial shampoos containing ZPT were deposited on disks with nitrocellulose filter paper and artificial skin substrate (Vitro-Skin) even after rinse-off, so zone of inhibition were observed.
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KEYWORD
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anti-dandruff activity, disk diffusion test, shampoo, Malassezia
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